A. Jakubowiak et al., Inhibition of the transforming growth factor beta 1 signaling pathway by the AML1/ETO leukemia-associated fusion protein, J BIOL CHEM, 275(51), 2000, pp. 40282-40287
The t(8;21) translocation, found in adult acute myelogenous leukemia, resul
ts in the formation of an AML1/ETO chimeric transcription factor. AML1/ETO
expression leads to alterations in hematopoietic progenitor cell differenti
ation, although its role in leukemic transformation is not clear. The N-ter
minal portion of AML1, which is retained in AML1/ETO, contains a region of
homology to the FAST proteins, which cooperate with Smads to regulate trans
forming growth factor beta1 (TGF-beta1) target genes. We have demonstrated
the physical association of Smad proteins with AML1 and AML1/ETO by immunop
recipitation and have mapped the region of interaction to the rant homology
domain in these AML1 proteins. Using confocal microscopy, we demonstrated
that AML1, and ETO and/or AML1/ETO, colocalize with Smads in the nucleus of
t(8;21)-positive Kasumi-1 cells, in the presence but not the absence of TG
F-beta1. Using transient transfection assays and a reporter gene construct
that contains both Smad and AML1 consensus binding sequences, we demonstrat
ed that overexpression of AML1B cooperates with TGF-beta1 in stimulating re
porter gene activity, whereas AML1/ETO represses basal promoter activity an
d blocks the response to TGF-beta1. Considering the critical role of TGF-be
ta1 in the growth and differentiation of hematopoietic cells, interference
with TGF-beta1 signaling by AML1/ETO may contribute to leukemogenesis.