RNA polymerase - Promoter recognition. Specific features of electrostatic potential of "early" T4 phage DNA promoters

Comparative analysis of electrostatic potential distribution for "early" T4 phage promoters was undertaken, along with calculation of topography of el ectrostatic potential around the native and ADP-ribosylated C-terminal doma in of RNA polymerase cc-subunit. The data obtained indicate that there is s pecific difference in the patterns of electrostatic potential distribution in far upstream regions of T4 promoters differing by their response to ADP- ribosylation of RNA polymerase. A specific change in profiles of electrosta tic potential distribution for the native and ADP-ribosylated forms of RNA polymerase or-subunit was observed suggesting that this factor may be respo nsible for modulating T4 promoter activities in response to the enzyme modi fication.