Process development for industrial-scale preparation of carboxypeptidase Ysecreted by Saccharomyces cerevisiae

Authors
Shiba, Y Kanari, Y Fukui, F Yoshikawa, H
Citation
Y. Shiba et al., Process development for industrial-scale preparation of carboxypeptidase Ysecreted by Saccharomyces cerevisiae, J BIOSCI BI, 90(4), 2000, pp. 470-472
Citations number
12
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
ISSN journal
13891723 → ACNP
Volume
90
Issue
4
Year of publication
2000
Pages
470 - 472
Database
ISI
SICI code
1389-1723(200010)90:4<470:PDFIPO>2.0.ZU;2-8
Abstract
A method for industrial-scale preparation of carboxypeptidase Y (CPY), whic h was secreted by Saccharomyces cerevisiae KS58-2D/pCY303 into the culture broth, was developed. Because the purification process consists of a few si mple unit operations including only one chromatography step, a higher CPY r ecovery was achieved than that in the process using disrupted baker's yeast . Approximately 100 g of purified CPY powder was constantly obtained using the final culture broth from a 500-l fermenter.