Y. Shiba et al., Process development for industrial-scale preparation of carboxypeptidase Ysecreted by Saccharomyces cerevisiae, J BIOSCI BI, 90(4), 2000, pp. 470-472
A method for industrial-scale preparation of carboxypeptidase Y (CPY), whic
h was secreted by Saccharomyces cerevisiae KS58-2D/pCY303 into the culture
broth, was developed. Because the purification process consists of a few si
mple unit operations including only one chromatography step, a higher CPY r
ecovery was achieved than that in the process using disrupted baker's yeast
. Approximately 100 g of purified CPY powder was constantly obtained using
the final culture broth from a 500-l fermenter.