Anti-oxidant effect of Withania somnifera glycowithanolides in chronic footshock stress-induced perturbations of oxidative free radical scavenging enzymes and lipid peroxidation in rat frontal cortex and striatum

The antioxidant activity of Withania somnifera (WS) glycowithanolides was a ssessed in chronic footshock stress induced changes in rat brain frontal co rtex and striatum. The stress procedure, given once daily for 21 days, indu ced an increase in superoxide dismutase (SOD) and lipid peroxidation (LPO) activity, with concomitant decrease in catalase (CAT) and glutathione perox idase (GPX) activities in both the brain regions. WS glycowithanolides (WSG ), administered orally 1 h prior to the stress procedure for 21 days, in th e doses of 10, 20 and 50 mg/kg, induced a dose-related reversal of the stre ss effects. Thus, WSG tended to normalise the augmented SOD and LPO activit ies and enhanced the activities of CAT and GPX. The results indicate that, at least part of chronic stress-induced pathology may be due to oxidative s tress, which is mitigated by WSG, lending support to the clinical use of th e plant as an antistress adaptogen. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.