Selective chemiluminescence analysis of Amadori form of glycated human serum albumin

Human serum albumin was purified by size-exclusion liquid chromatography. T he glycated albumin in the effluent was selectively measured by a post-colu mn reaction detector. The selective reaction mechanism is to measure the am ount of Amadori form of glycated albumin. According to the Maillard reactio n, glycosylated amines are rearranged to Amadori form ketoamines which can produce superoxide in strong alkaline solution, and this superoxide produce s chemiluminescence with lucigenin. Tbe chemiluminescence intensity was rel ated to the concentration of glycated albumin in albumin while the fluoresc ence intensity was the same.