A random amplified polymorphic DNA polymerase chain reaction technique that differentiates between Neospora species

Neospora caninum is a recently described coccidial parasite that was first isolated from a dog in 1988 and has subsequently been shown to infect a wid e range of mammals. Neospora hughesi, a new species of this genus, has rece ntly been isolated from the spinal cord of horses showing clinical signs of equine protozoal myeloencephalitis. The random amplified polymorphic DNA p olymerase chain reaction technique is capable of differentiating between N. caninum and N. hughesi.