Vl. Sdepanian et al., Assessment of gliadin in supposedly gluten-free foods prepared and purchased by celiac patients, J PED GASTR, 32(1), 2001, pp. 65-70
Citations number
26
Categorie Soggetti
Pediatrics,"Medical Research General Topics
Journal title
JOURNAL OF PEDIATRIC GASTROENTEROLOGY AND NUTRITION
Background: The present study was designed to evaluate the presence of glia
din in homemade foods prepared by patients with celiac disease and/or their
relatives, as well as in processed products consumed by such patients in S
ao Paulo, Brazil, by enzyme immunoassay (EIA) and Western blot (WB) analysi
s.
Methods: One hundred ninety samples were analyzed: 108 homemade foods prepa
red in homes of patients with celiac disease, 81 processed products, and 1
positive control of homemade food. All samples were analyzed by EIA based o
n monoclonal antibodies to heat stable omega -gliadins and related prolamin
s from wheat. rye, and barley. Samples were also analyzed using the WE tech
nique.
Results: Only one (0.9%) of 108 homemade foods contained detectable amounts
of gliadin, as determined by EIA. Twelve of 81 processed products containe
d gliadin by EIA, as follows: 5 of 61 without gluten listed in the ingredie
nts, 2 of 11 malt extracts, 1 of 2 wheat starches, 1 of 2 types of beer. an
d all 3 positive control products. Gliadin content of these products was be
tween 4 and 10 mg of gliadin/100 g of product, except for the wheat starch
sample (28 mg of gliadin/100 g) and all 3 samples with gluten (>4000 mg of
gliadin/100 g). The positive control of homemade food contained 152 mg of g
liadin/100 g. One hundred three of 190 samples were analyzed by WE, and 21
of these were gliadin positive. A comparison of results obtained by EIA and
WE showed no statistical differences between the methods.
Conclusions: The greater part of the foods prepared in homes of patients wi
th celiac disease and most processed products supposed to be gluten-frer di
d not contain gliadin. Therefore, celiac patients adequately prepare gluten
-free homemade food and have the expertise to purchase processed gluten-fre
e food in Sao Paulo, Brazil.