Cytotoxicity of 4-hydroxy-2E-hexenal, a lipid peroxidation-derived aldehyde, and changes of its content in frozen yellowtail meat

Cytotoxicity of 4-hydroxy-2E-hexenal (HHE), a hepatotoxic aldehyde, to Chin ese hamster lung fibroblast V79 and Balb/c mouse semi-normal cell line BALB /3T3 clone A31 cells was examined. The 50%-inhibitory concentrations to V79 and A31 were 3 mu mol/L and 16 mu mol/L, respectively. Judging from the se nsitivity, HHE might show cytotoxicity below these concentrations. Changes in HHE and malonaldehyde (MA) contents were also examined in yellowtail mea t stored at -20 degreesC. HHE content increased up to 60.82+/-4.39 mu mol/k g meat during 28 weeks of storage. In contrast to HHE, MA content increased only slightly in the meat during 28 weeks of storage. From the viewpoint o f food hygiene, it is noteworthy that the HHE content increased even when m eat was stored at -20 degreesC.