T. Sakai et al., Cytotoxicity of 4-hydroxy-2E-hexenal, a lipid peroxidation-derived aldehyde, and changes of its content in frozen yellowtail meat, J FOOD HYG, 41(6), 2000, pp. 368-370
Cytotoxicity of 4-hydroxy-2E-hexenal (HHE), a hepatotoxic aldehyde, to Chin
ese hamster lung fibroblast V79 and Balb/c mouse semi-normal cell line BALB
/3T3 clone A31 cells was examined. The 50%-inhibitory concentrations to V79
and A31 were 3 mu mol/L and 16 mu mol/L, respectively. Judging from the se
nsitivity, HHE might show cytotoxicity below these concentrations. Changes
in HHE and malonaldehyde (MA) contents were also examined in yellowtail mea
t stored at -20 degreesC. HHE content increased up to 60.82+/-4.39 mu mol/k
g meat during 28 weeks of storage. In contrast to HHE, MA content increased
only slightly in the meat during 28 weeks of storage. From the viewpoint o
f food hygiene, it is noteworthy that the HHE content increased even when m
eat was stored at -20 degreesC.