ENANTIOMERIC SEPARATION OF SOME PIPERIDINE-2,6-DIONE DRUGS ON TOLYLCELLULOSE BY LIQUID-CHROMATOGRAPHY

The direct liquid chromatographic resolution of a group of drugs, all having a piperidine-2,6-dione structure in common was investigated usi ng a cellulose-based chiral stationary phase. The experimental tris(4- methylbenzoate) cellulose column (Bio-Rad RSL, Belgium) has the polyme ric layer covalently bonded onto an aminopropylated silica support. Re versed phase as well as normal phase conditions were applied without d eterioration of the column. Aminoglutethimide and glutethimide were ea sily resolved using methanol-water mixtures as mobile phase. Acetylami noglutethimide, the major metabolite of aminoglutethimide, was separat ed from the parent drug but its enantiomers were not completely resolv ed. Thalidomide enantiomers were at best resolved under normal phase c onditions with n-hexane as the major constituent of the mobile phase. No chiral interaction on this column was noticed for 3-phenylacetylami nopiperidine-2,6-dione.