Prooxidant role of histidine in hypoxic stressed mice and Fe3+-induced lipid peroxidation

An attempt was made to study the effect of histidine on reactive oxygen spe cies in a rodent model of hypoxic stress and in Fe3+-ascorbic acid induced lipid peroxidation in mouse brain homogenates. The latency for onset of hyp oxic stress-induced convulsions was decreased in histidine-treated animals with a concomitant rise in brain lipid peroxidation levels. In vitro, histi dine potentiated Fe3+-ascorbic acid-indirected lipid peroxidation irt mouse brain homogenates while other antioxidants like B-HT and U-74500A inhibite d the same. Moreover; Fe3+-histidine-induced lipid peroxidation could nor b e inhibited by preincubation for the system with high concentrations of asc orbic acid. Thus, it is concluded that histidine nets as a strong prooxidan t potentiating the genesis of reactive oxygen species during hypoxic stress as well as during Fe3+-ascorbic acid-induced lipid peroxidation. (C) 2000 Prous Science. All rights reserved.