Recombinant components of the EcoRII restriction-modification system: Restriction endonuclease can interact with DNA center dot RNA duplexes

Authors
Babkina, OV Evstafieva, AG Chichkova, NV Vartapetian, AB Muller, S Baskunov, VB Petrauskene, OV Kochetkov, SN Gromova, ES
Citation
Ov. Babkina et al., Recombinant components of the EcoRII restriction-modification system: Restriction endonuclease can interact with DNA center dot RNA duplexes, MOL BIOL, 34(6), 2000, pp. 913-920
Citations number
25
Categorie Soggetti
Molecular Biology & Genetics
Journal title
MOLECULAR BIOLOGY
ISSN journal
00268933 → ACNP
Volume
34
Issue
6
Year of publication
2000
Pages
913 - 920
Database
ISI
SICI code
0026-8933(200011/12)34:6<913:RCOTER>2.0.ZU;2-M
Abstract
To obtain recombinant restriction endonuclease (R) and methylase (M) of the EcoRII restriction-modifcation system, bacterial strains overproducing the ir functional hexahistidine derivatives were constructed. Active full-lengt h R EcoRII was produced only in cells that also expressed M.EcoRII from a m ulticopy plasmid. Recombinant R EcoRII bound with hybrid DNA RNA duplexes.