Monitoring gene flow from transgenic sugar beet using cytoplasmic male-sterile bait plants

One of the most discussed environmental effects associated with the use of transgenic plants is the flow of genes to plants in the environment. The fl ow of genes may occur through pollen since it is the reproductive system th at is designed for gene movement. Pollen-mediated gene escape is hard to co ntrol in mating plants. Pollen from a wind pollinator can move over distanc es of more than 1000 m. To investigate the efficiency of transgenic pollen movement under realistic environmental conditions, the use of bait plants m ight be an effective tool. In this study, cytoplasmic male-sterile (CMS) su gar beets were tested with regard to their potential for monitoring transge ne now. As the pollen source, transgenic sugar beets were used that express recombinant DNA encoding viral (beet necrotic yellow vein virus) resistanc e, and antibiotic (kanamycin) and herbicide (glufosinate) tolerance genes. In a field trial, the effectiveness of a hemp (Cannabis sativa) stripe cont ainment strategy was tested by measuring the frequency of pollinated CMS ba it plants placed at different distances and directions from a transgenic po llen source. The results demonstrated the ineffectiveness of the containmen t strategy. Physiological and molecular tests confirmed the escape and prod uction of transgenic offspring more than 200 m behind the hemp containment. Since absolute containment is unlikely to be effective, the CMS-bait plant detection system is a useful tool for other monitoring: purposes.