Storage of horse blood in different additive solutions at 22 degrees and 4degrees C

Blood was drawn from 16 healthy, adult hones. The preservation of this bloo d took place in form of combining either whole blood with a CPDA-Stabilizer , erythrocyte-concentrate-1 (ERY-1) with a SAG-M-Stabilizer or erythrocyte- concentrate-2 (ERY-2) with a GM-Solution [free of chloride ions). Precede w e carried out a depletion or chloride ions in ERY-2. The hone blood units w ere stored for either 4 days at 22 degreesC or for 36 days at 4 degreesC. W e then determined following parameters in this blood units: hematology (RBC , Hb(total), Hb(extracellular) rate of hemolysis, Hct, MCV, MCHC, MCH, frag ility of erythrocytes), hemoxymetry (pH, p50, O(2)Hb), electrolytes (sodium , potassium, chloride), enzyme (LDH), metabolites (glucose, lactate, 2,3-DP G, ATP). Concerning the hematological parameters there was a slight increase of MCV as well as a slight decrease of MCHC and ct storing time of only 5 weeks th e Hd increased. In total changes due to storage were more obvious at the st oring temperature of 4 degreesC: In whole blood and ERY-1 the pH level drop ped almost continiuously during the storing periode. In comparision to that the protone activity in ERY-2 didn't drop below the original level. The in crease of acidity in whole blood and ERY-1 caused a decrease of intracellul ar parameter 2,3-DPG. The concentration of 2,3-DPG in ERY-2 on the of her h and remained the same, without significant deviations. The results for pH a nd p50 correlated negatively with each other. The extracellular reduction o f sodium and increase of potassium as well as the rise in extracellular LDH allow the assumption of an impaired function of membraneous integrity The significant increase of maximal and minimal erythrocyte fragility indicated membraneous instability of the cells in whole blood and ERY-1 too, but not in ERY-2, The ATP concentration of the erythrocytes was relatively high in all preserves and remained unchanged. The average rate of hemolysis, <0,14 %, after 4 days of storage at 22<degrees>C maintained relatively smell. Onl y in ERY-2 it increased to on intolerable value of 1,01% after 36 days of s torage at 4 degreesC. In total the whole blood and ERY-1 were still suitable for a transfusion af ter storing periode of 4 days at a temperature of 22 degreesC as well as af ter storing time of 36 days at a temperature of 4 degreesC. In ERY-2 the ex tracellular potassium level increased unjustifiably on day 4 at a temperatu re of 22 degreesC (>50 mmol/l). Until day 29 during the storing periode of 5 weeks at 4 degreesC the ERY-2 was superior to the other preserves, due to the favourable progress of the erythrocytic vitality parameters.