Effects of ethanol on startle responding in alcohol-preferring and -non-preferring rats

The objectives of the present study were to determine (a) if differences ex ist between the selectively bred alcohol-preferring (P) and -nonpreferring (NP) lines of rats in the acoustic startle response (ASR) and prepulse inhi bition (PPI), and (6) the effects of ethanol on these measures. Alcohol-nai ve adult female P and NP rats received a single i.p. injection of saline or ethanol (0.25, 0.5, 1.0, or 1.5 g/kg) and were placed in the startle appar atus 10 min later. After a 5-min acclimation period, rats received five alt ernating trials of a startle stimulus alone (SSA) (115-dB white noise) or a PPI trial (90-dB white noise preceding a 115-dB white noise). Analysis of the ASR revealed that P rats exhibited higher startle amplitudes than did N P rats with saline injections. The 0.5-g/kg ethanol dose reduced the startl e amplitude in P, but not NP, rats. The 1.0- and 1.5-g/kg ethanol doses nea rly abolished the ASR in the NP line, whereas only the highest ethanol dose had this effect in the P line. Vehicle-heated P and NP rats exhibited comp arable PPI levels, but only P rats showed a significant disruption (30%) at the 0.50-g/kg ethanol dose. Neither P nor NP rats were affected by ethanol treatment at the 0.25-g/kg dose. Overall, the results suggest that: (a) th e difference in baseline ASR may indicate line differences in the neurocirc uitry mediating this response, possibly reflecting higher innate levels of emotional reactivity in the P line; (b) the P line may be more sensitive th an the NP line to the effects of ethanol in reducing emotional reactivity; and (c) low-dose ethanol may have a greater disruptive effect on sensorimot or gating mechanisms in the P than NP rat. (C) 2000 Elsevier Science Inc. A ll rights reserved.