Change of immunoreactive P-selectin was examined in rat brain after tr
ansient middle cerebral artery (MCA) occlusion (O) with anti-P-selecti
n monoclonal antibody using brain samples of sham control and after is
chemia. Temporal, spatial, and cellular changes of immunohistochemical
expressions of P-selectin were evaluated with rat brain sections at 2
and 8 h, 1, 3, and 7 days of reperfusion after 1 h of MCAO. Western b
lot showed a single band at molecular weight of 140 kDa for P-selectin
after ischemia. P-selectin immunoreactivity was not normally present
in rat brain sections. However, it was expressed mainly in the post-ca
pillary venules of the cerebral cortex and caudate in the MCA territor
y with a peak at 8 h-l day. The expression was diminished by 3 days of
reperfusion. The present results indicate that P-selectin was express
ed from an earlier stage of reperfusion in post-capillary venules, and
the expression became maximum at the same time both in the cerebral c
ortex and caudate. (C) 1997 Elsevier Science Ireland Ltd.