Cardamine amara is represented in the Iberian Peninsula by two subspecies:
the diploid (2n = 16) subsp. pyrenaea, occurring in the Eastern Pyrenees, a
nd the tetraploid (2n = 32) subsp. olotensis, concentrated at the lower alt
itudes in Catalonia, N and NE of Barcelona. Morphometric analysis of popula
tions of these taxa and four other subspecies of C. amara, which occur in o
ther parts of Europe, supported their subspecific distinctness. RAPD (Rando
m Amplified Polymorphic DNA) analysis of all six European subspecies of C.
amara provided more evidence supporting this taxonomic treatment. Lectotype
s were designated for the following names: C. amara var. parviflora Cadeval
l, C. amara subsp. pyrenaea Sennen, C. amara subsp. siifolia Sennen, and C.
amporitana Sennen & Pau.