Ef. Berrios et al., AFLP mapping of QTLs for in vitro organogenesis traits using recombinant inbred lines in sunflower (Helianthus annuus L.), THEOR A GEN, 101(8), 2000, pp. 1299-1306
Genetic control for two in vitro organogenesis traits, the number of shoots
per explant plated (S/E) and the number of shoots per regenerating explant
(S/RE), was investigated in 75 recombinant inbred lines (RILs) of sunflowe
r and their two parents (PAC-2 and RHA-266). Genetic variability was observ
ed among the 75 Rns for the organogenesis traits studied. Some RILs present
ed significant differences when compared with the best parental line (RHA-2
66). Genetic gain, in terms of the percentage of the best parent, for 32% o
f the selected Rns was significant. A set of 99RILs from the same cross inc
luding the 75 mentioned above was screened with 333 AFLP markers and a link
age map was constructed based on 264 linked loci. Six putative QTLs for the
S/RE (tentatively named osl) and seven QTLs for the S/E (ose) trait were d
etected using composite interval mapping. For each trait, the QTLs explaine
d 52% (ose) and 67% (osr) of the total phenotypic variance. These results s
uggested that additive gene effects predominate in explaining a large propo
rtion of the observed genetic variation associated with regeneration abilit
y. The coincidental location of QTLs for S/E and S/RE is discussed.