identification of an introduced bovine herpesvirus type 1 strain in a closed dairy herd by experimental virus reactivation followed by DNA restriction enzyme analysis
Whm. Van Der Poel et al., identification of an introduced bovine herpesvirus type 1 strain in a closed dairy herd by experimental virus reactivation followed by DNA restriction enzyme analysis, TIJD DIERG, 125(23), 2000, pp. 714-717
In a closed dairy herd in the province of Utrecht in 1995, nine replacement
heifers were erroneously intramuscularly vaccinated with Tracherine(R), a
live virus IBR vaccine. More than 18 months later, serology of the herd rev
ealed that a large part of the herd had developed an antibody response towa
rds BHV1 (62 of 87 animals). To investigate whether Tracherine(R) had recir
culated on the farm, four BHV1 antibody positive animals, of which two had
been vaccinated with Tracherine(R), were treated with corticosteroids to re
activate latent BHV1. Two virus isolates were obtained and subsequently ana
lysed by restriction enzyme analysis. Both isolates were identified as BHV1
.1 subtypes. One of the isolates was clearly distinct from Tracherine(R) an
d was most likely a BHV1 field virus. A BHV1 field virus was most likely in
troduced into the farm even though the herd was closed, the animals had not
been in contact with other cattle, and preventive hygienic measures had be
en implemented. There was no indication that Tracherine(R) had recirculated
.