RAPD analysis of genetic variation among propanil-resistant and -susceptible Echinochloa crus-galli populations in Arkansas

The extensive use of propanil for weed control in Oryza sativa over the pas t 30 yr has led to the development of propanil-resistant Echinochloa crus-g alli. This problem was first identified in one Arkansas county in 1990. Sin ce that time, 171 resistant populations have been identified in 20 counties . Random amplified polymorphic DNA (RAPD) analysis was used to assess genet ic variation among populations of propanil-resistant and -susceptible E. cr us-galli in Arkansas and to elucidate the origin of resistance and its disp ersal. Sixteen E, crus-galli populations from seven Arkansas counties and o ne population from Mississippi were analyzed using 13 primers, which amplif ied a total of 159 repeatable fragments. Genetic distance was calculated am ong the populations, and the populations were clustered. The analysis revea led two distinct clusters with an average between-cluster genetic distance of 0.436 and average within-cluster distances of 0.012 and 0.029. These clu sters may represent different Echinochloa species. Each cluster contained a t least one resistant population and populations from different regions of the state. In addition, several resistant populations from different: regio ns of the state were genetically nearly identical. Thus, it is likely that resistant biotypes have spread in Arkansas by seed dispersal and by indepen dent mutation events. This suggests that there are multiple origins of the resistance, and simple control of weed seed dispersal will not stop the spr ead of resistance.