Strip transects located along roads are commonly used to estimate waterfowl
populations and characterize associated wetland habitat. We used data coll
ected in May and early June, 1995, on forty-five 40-km(2) plots in North Da
kota to evaluate bias of 800-m and 400-m wide roadside transects for sampli
ng wetlands relative to a larger (40-km(2)) scale and to compare duck abund
ance at the two widths. Densities of all basins combined and of seasonal ba
sins considered alone were biased high for both transect widths, but mean b
ias did not differ from zero for temporary or semipermanent basins. Biases
did not occur when excavated seasonal and temporary basins (i.e., road ditc
hes) were excluded from the sample. Mean basin density was higher for the i
nner (400-m) transect width than for the outer transect width (area remaini
ng of the 800-m transect, outside of center 400-m width) for all basins com
bined and for seasonal and temporary basins. We detected an area-related bi
as in the occurrence of basins in transects: smaller basins (0.08-1.6 ha) w
ere over-represented in transect samples by 2.9-6.5%, and larger basins (gr
eater than or equal to 11 ha) were under-represented in 800-m transects by
7.3% and in 400-m transects by 16.3%. We compared the distribution of ducks
relative to water conditions in the inner and outer transect widths to eva
luate whether they were affected by proximity to the road. Mallards (Anas p
latyrhynchos L.), northern pintails (A. acuta L.), and gadwall (A. strepera
L.) responded to water conditions equally in the inner and outer transect
widths, but northern shovelers (A. clypeata L.) and blue-winged teal (A, di
scors L.) responded more strongly to wetlands on the inner than the outer t
ransect width, indicating that estimates of these species would be higher f
rom a 400-m wide transect than from an 800-m wide transect. Differences in
an adjustment index, used to account for the portion of basin obscured from
view, were highly variable between inner and outer transect widths but did
not indicate that use of wider transects was hampered by visibility. Biase
s of transect sampling need to be carefully considered when extrapolating w
etland basin or duck densities from transects to larger landscape scales.