Treatment of cervicitis is associated with decreased cervical shedding of HIV-1

Objective: To determine whether cervical mucosal shedding of HIV-1 RNA and HIV-1 infected cells decreases following successful treatment of cervicitis . Design: Prospective interventional study. Setting: Sexually Transmitted infections Clinic, Coast Provincial General H ospital, Mombasa, Kenya. Participants: Thirty-six HIV-1 seropositive women with cervicitis: 16 with Neisseria gonorrhoeae, seven with Chlamydia trachomatis, and 13 with non-sp ecific cervicitis. Interventions: Treatment of cervicitis. Main outcome measures: Levels of total (cell-free and cell-associated) HIV- 1 RNA and presence of HIV-1 DNA (a marker for infected cells) in cervical s ecretions before and after resolution of cervicitis. Results: After treatment of cervicitis, the median HIV-1 RNA concentration in cervical secretions was reduced from 4.05 to 3.24 log(10) copies/swab (P = 0.001). Significant decreases in cervical HIV-1 RNA occurred in the subg roups with N. gonorrhoeae (3.94 to 3.28 log(10) copies/swab; P = 0.02) and C. trachomatis (4.21 to 3.29 log(10) copies/swab; P = 0.02). Overall, the p revalence of HIV-1 infected cells in cervical secretions also decreased aft er treatment, from 67% to 42% (odds ratio, 2.8; 95% confidence interval, 1. 3-6.0; P = 0.009). Detection of infected cells was associated with higher m ean HIV-1 RNA levels (4.04 versus 2.99 log(10)copies/swab; P < 0.0001). Conclusions: Effective treatment of cervicitis resulted in significant decr eases in shedding of HIV-1 virus and infected cells in cervical secretions. Treatment of sexually transmitted diseases may be an important means of de creasing the infectivity of HIV-1 seropositive women by reducing exposure t o HIV-1 in genital secretions. (C) 2001 Lippincott Williams & Wilkins.