This article summarizes the results of a fungal investigation of a new, sev
en-story, 170-room hotel. The owners of the building were refused an occupa
ncy permit by local building officials because of concerns about fungal con
tamination. This article discusses the processes that were used to investig
ate and abate the fungal concerns in this building. This investigation invo
lved comparing fungal infestation levels in various building materials; inc
luding two different types of gypsum wallboard. During initial testing the
indoor air concentrations of problematic fungi were up to 50 times higher t
han outdoor levels. After the removal of the fungal-infested materials and
the replacement with more acceptable materials, the indoor air concentratio
n of problematic fungi dropped to less than one-fourth of outdoor levels.