Fluorescent labeling of phenol using 8-(4,6-dichloro-1,3,5-triazinylamino)quinoline

8-(4,6-Dichloro-1,3,5-triazinylamino)quinoline (DTAQ), a new fluorescent re agent for determining phenol, was synthesized by reacting 8-aminoquinoline with cyanuric chloride at 0 degreesC and its properties were studied. Based on the excitation and emission peak shifts from 334 and 443 nm to 361 and 485 nm, respectively, when DTAQ reacted with phenol in alkaline medium, a s imple and selective method for the spectrofluorimetric determination of phe nol has been established. The relative S.D. of the method was found to be 3 .7% by 9 replicate determinations of 1.7 x 10(-7) g ml(-1) phenol solution. The linear range is 1.7 x 10(-7)-1.3 x 10(-5) g ml(-1) with a detection li mit of 1.1 x 10(-8) g ml(-1) (S/N = 3). The method has been successfully us ed to determine phenols in industrial wastewater. The results obtained were in good agreement with those obtained by using 4-aminoantipyrine colorimet ry. (C) 2001 Elsevier Science B.V. All rights reserved.