Pantoea agglomerans strain EH318 produces two antibiotics that inhibit Erwinia amylovora in vitro

Citation
Sai. Wright et al., Pantoea agglomerans strain EH318 produces two antibiotics that inhibit Erwinia amylovora in vitro, APPL ENVIR, 67(1), 2001, pp. 284-292
Citations number
57
Categorie Soggetti
Biology,Microbiology
Journal title
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
ISSN journal
00992240 → ACNP
Volume
67
Issue
1
Year of publication
2001
Pages
284 - 292
Database
ISI
SICI code
0099-2240(200101)67:1<284:PASEPT>2.0.ZU;2-4
Abstract
Pantoea agglomerans (synonym: Erwinia herbicola) strain Eh318 produces thro ugh antibiosis a complex zone of inhibited growth in an overlay seeded with Erwinia amylovora, the causal agent of fire blight, This zone is caused by two antibiotics, named pantocin A and B. Using a genomic library of Eh318, two cosmids, pCPP702 and pCPP704, were identified that conferred on Escher ichia coli the ability to inhibit growth of E. amylovora. The two cosmids c onferred different antibiotic activities on E. coil DH5 alpha. and had dist inct restriction enzyme profiles. A smaller, antibiotic-conferring DNA segm ent from each cosmid was cloned, Each subclone was characterized and mutage nized with transposons to generate clones that were deficient in conferring pantocin A and B production, respectively. Mutated subclones were introduc ed into Eh318 to create three antibiotic-detective marker exchange mutants: strain Eh421 (pantocin A deficient); strain Eh439 (pantocin B deficient), and Eh440 (deficient in both pantocins), Cross-hybridization results, restr iction maps, and spectrum-of-activity data using the subclones and marker e xchange mutants, supported the presence of two distinct antibiotics, pantoc in A and pantocin B, whose biosynthetic genes were present in pCPP702 and p CPP704, respectively. The structure of pantocin A is unknown, whereas that of pantocin B has been determined as (R)-N-[((S)-2-amino-propanoylamino)-me thyl]-2-methanesulfonyl-succinamic acid. The two pantocins mainly affect ot her enteric bacteria, based on limited testing.