Effect of melatonin on the pulsatile luteinizing hormone and growth hormone secretion in ewe lambs under food restriction

The aim of the present work was to characterize the pulsatile LH and GH sec retion in food restricted Suffolk ewe lambs (n=5, R-MEL group) receiving da ily oral administration of melatonin (3mg/20 mL, at 16:00 h), at 20-26- and 30 weeks of age, after 4,10 and 14 weeks of food restriction. Melatonin tr eatment was initiated at 10 weeks of age. Food restricted lambs of the same age, without melatonin treatment acted as comparative group (n=6, R-SOL). Blood samples were collected from a jugular vein by means of an indwelling catheter at 10 min-intervals for 6 hours, from 09:00 h. LH and GH were meas ured by radioimmunoassay. Plasma concentrations of both hormones were analy zed by the Cluster program to define pulse frequency (n degrees pulses/6h), and pulse amplitude (ng/mL). The transversal mean of LH and GH was also ca lculated (ng/mL/6h). In the R-MEL group, the transversal mean of LH tended to increase from 0.35+/-0.04 in 20 weeks old lambs to 0.61+/-0.15 ng/mL/6h in 30 weeks old lambs while remained unchanged in the R-SOL group: 0.34+/-0 .03 and 0.25+/-0.05 ng/mL/6h, respectively. LH pulse frequency did not chan ge and was not different between both groups. LH pulse amplitude tended to be higher in R-MEL than in R-SOL lambs at 30 weeks of age: 0.76+/-0.28 and 0.38 0.09 ng/mL, respectively (P<0.07). In contrast to the low effect of me latonin on LH secretion, the transversal mean and the GH pulse amplitude we re significantly modified by the melatonin treatment. R-MEL lambs had mean concentrations of 3.28+/-1.14, 6.25+/-1.88 and 7.26+/-1.57 ng/mL/6h at 20,2 6 and 30 weeks, respectively while R-SOL lambs had 15.69+/-1.37 (P<0.01), 1 3.25+/-2.66 (P<0.02) and 19.12+/-3.88ng/mL/6h (P<0.03), respectively. Pulse amplitude was also lower in R-MEL than in R-SOL lambs at 20, 26 and 30 wee ks of age. GH pulse frequency was not different between groups and ages. Re sults suggest that melatonin is involved in the control of GH secretion in growing sheep, overpassing the metabolic effect of food restriction on GH s ecretion. However, in the case of LH, the metabolic impact of food restrict ion is not completely overcome by the stimulatory effect of melatonin on th e LHRH/LH secretion, suggesting that the metabolic status of the growing sh eep should be in concert with the photoperiod to coordinate the initiation of puberty.