Field strains of Ornithobacterium rhinotracheale were tested on their virul
ence in different chicken breeds. Ornithobacterium rhinotracheale was able
to induce lesions after aerosol challenge without a previous priming with v
irus, and thus O. rhinotracheale was proven to be a primary pathogen. The v
irulence of Dutch strains, isolated between 1995 and 1998, did not increase
, but the Dutch isolates and a South African strain were more pathogenic co
mpared with an American strain of O. rhinotracheale. White specific-parhoge
n-free leghorns were less susceptible to O. rhinotracheale infection than b
roilers, whereas there was no difference in susceptibility between commerci
al broilers and specific-parhogen-free broilers.