Immuno and cytochemical localization of Trypanosoma cruzi nitric oxide synthase

The localization and subcellular distribution of Trypanosoma cruzi nitric o xide synthase was investigated in epimastigote cells by immunocytochemistry at electron and light microscope level, using a polyclonal antibody to neu ronal nitric oxide synthase, and also, at light microscope level, by the ni cotinamide adenine dinucleotide phosphate-diaphorase histochemical reaction . The immunoreactivity was ultrastructurally localized by electron microsco py in the inner surface of cell membranes and in free cytosolic clusters in the body, flagellum and apical extreme. Light microscopy showed that immun oprecipitates, specific for the Trypanosoma cruzi nitric oxide synthase, co -localized with the formazan precipitates generated by the diaphorase react ion in the same areas identified by electron microscopy. These results, tak en together with previous finding from our laboratory could help to explain the involvement of the nitric oxide transduction pathway in T. cruzi epima stigote motility.