Identification of partially deuterated exometabolites during cultivation of tea fungus in heavy water

Exometabolites of tea fungus during the cultivation on heavy water were stu died by methods of high-resolution proton and deuterium exchange. Is was sh own that, depending on the degree of exchange of proton for deuterium in th e methyl group of acetate, three isotopomers exist: -CH3, -CH2D, and -CHD2. Ethanol is represented by six isotopomers: -CHD-CH3, -CD2-CH3, -CD2 -CH2D, -CD2-CHD2, -CHD-CH2D, and -CHD-CHD2. Relative concentration of these isoto pomers and the percent content of deuterium were determined. Based on these data, the kinetics of metabolism of the object cultivated on heavy water w as analyzed. It was show that the efficiency of glucose utilization is the same as during cultivation on light water with the exception that the time of utilization is significantly greater. At the same time, the parameters o f utilization of ethanol and its conversion into acetic acid in the life ac tivity of the tea fungus in heavy and light water are very similar.