Er. Gouveia et al., Optimisation of medium composition for clavulanic acid production by Streptomyces clavuligerus, BIOTECH LET, 23(2), 2001, pp. 157-161
Among four different commercially available nitrogen sources containing soy
bean derivatives, a protein extract of soybean gave the highest yield for c
lavulanic acid production by Streptomyces clavuligerus. A statistical metho
d based on factorial design of experiments was applied to optimise the medi
um. An empirical model was obtained by applying response surface statistica
l analysis. The analysis of variance showed that concentrations of protein
extract of soybean and glycerol and the interaction between these two varia
bles were significant at 95% level of confidence. The maximum clavulanic ac
id concentration obtained in 72 h was 1.2 g l(-1).