Application of (S)-N-(4-nitrophenoxycarbonyl) phenylalanine methoxyethyl ester as a new chiral derivatizing agent for proteinogenic amino acid analysis by high-performance liquid chromatography

Authors
Peter, A Vekes, E Torok, G
Citation
A. Peter et al., Application of (S)-N-(4-nitrophenoxycarbonyl) phenylalanine methoxyethyl ester as a new chiral derivatizing agent for proteinogenic amino acid analysis by high-performance liquid chromatography, CHROMATOGR, 52(11-12), 2000, pp. 821-826
Citations number
10
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
CHROMATOGRAPHIA
ISSN journal
00095893 → ACNP
Volume
52
Issue
11-12
Year of publication
2000
Pages
821 - 826
Database
ISI
SICI code
0009-5893(200012)52:11-12<821:AO(PME>2.0.ZU;2-C
Abstract
The application of (S)-N-(4-nitrophenoxycarbonyl)phenylalanine methoxyethyl ester, (S)-NIFE, as a new chiral derivatizing agent for the resolution of compounds possessing an amino group is described. Its applicability is demo nstrated by the resolution of proteinogenic amino acid enantiomers. The dia stereomeric derivatives produced were separated by reversed-phase high-perf ormance liquid chromatography. The effects of pH, excess reagent and reacti on time on the derivatization kinetics, and the effects of pH and the organ ic modifier on the separation, were investigated.