Immunodiagnosis of human fascioliasis by an enzyme-linked immunosorbent assay (ELISA) and a micro-ELISA

Authors
Carnevale, S Rodriguez, MI Santillan, G Labbe, JH Cabrera, MG Bellegarde, EJ Velasquez, JN Trgovcic, JE Guarnera, EA
Citation
S. Carnevale et al., Immunodiagnosis of human fascioliasis by an enzyme-linked immunosorbent assay (ELISA) and a micro-ELISA, CL DIAG LAB, 8(1), 2001, pp. 174-177
Citations number
27
Categorie Soggetti
Immunology
Journal title
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY
ISSN journal
1071412X → ACNP
Volume
8
Issue
1
Year of publication
2001
Pages
174 - 177
Database
ISI
SICI code
1071-412X(200101)8:1<174:IOHFBA>2.0.ZU;2-V
Abstract
Enzyme-Linked immunosorbent assay (ELISA) and micro-ELISA were evaluated fo r their ability to detect anti-Fasciola hepatica antibodies in humans by us ing excretory-secretory antigen. The sensitivity of each method was 100%, b ut the specificity was 100% for ELISA and 97% for micro-ELISA. The micro-EL ISA could be used as a screening assay and ELISA could be used as a confirm atory method for the serodiagnosis of human fascioliasis.