Production of a mouse antiserum to Bacteroides fragilis enterotoxin using a recombinant enterotoxin precursor

Authors
Sandini, S d'Abusco, AS La Valle, R Pantosti, A
Citation
S. Sandini et al., Production of a mouse antiserum to Bacteroides fragilis enterotoxin using a recombinant enterotoxin precursor, CL DIAG LAB, 8(1), 2001, pp. 190-191
Citations number
16
Categorie Soggetti
Immunology
Journal title
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY
ISSN journal
1071412X → ACNP
Volume
8
Issue
1
Year of publication
2001
Pages
190 - 191
Database
ISI
SICI code
1071-412X(200101)8:1<190:POAMAT>2.0.ZU;2-Q
Abstract
The precursor of the Bacteroides fragilis metalloprotease enterotoxin was c loned and expressed in Escherichia coli, which was not able to process the precursor into the biologically active enterotoxin. Mouse antiserum elicite d to the recombinant precursor reacted with the purified enterotoxin and wi th a crude enterotoxin preparation from an enterotoxigenic strain. The anti serum neutralized the cytotoxic activity of the enterotoxin in HT-29 cells.