The AIDA autotransporter system is associated with F18 and Stx2e in Escherichia coli isolates from pigs diagnosed with edema disease and postweaning diarrhea

Citation
U. Niewerth et al., The AIDA autotransporter system is associated with F18 and Stx2e in Escherichia coli isolates from pigs diagnosed with edema disease and postweaning diarrhea, CL DIAG LAB, 8(1), 2001, pp. 143-149
Citations number
31
Categorie Soggetti
Immunology
Journal title
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY
ISSN journal
1071412X → ACNP
Volume
8
Issue
1
Year of publication
2001
Pages
143 - 149
Database
ISI
SICI code
1071-412X(200101)8:1<143:TAASIA>2.0.ZU;2-X
Abstract
Pathogenic Escherichia call strains are known to cause edema disease (ED) a nd postweaning diarrhea (PWD) in piglets. Although the exact mechanisms of pathogenicity that lead to ED-PWD remain to be elucidated, E,coli-borne Shi ga-like toxin and adhesion-mediating virulence factors such as F18 adhesin or F4 fimbriae are believed to play a central role in ED-PWD, In light of t hese observations we investigated whether another E, call adhesin, the plas mid-encoded AII)A (adhesin involved in diffuse adherence) might also be pre sent in ED-PWD-causing E, coli isolates, For rapid screening for the AIDA s ystem in large numbers of isolates, a multiplex PCR method along with a dup lex Western blot procedure was developed. When screening 104 strains obtain ed from pigs with or without ED-PWD, we observed a high prevalence of the A IDA operon in porcine E, coli isolates, with over 25% of all strains being AIDA positive, and we could demonstrate a significant association of the in tact AIDA gene (orfB) with ED-PWD, while defects in orfB were associated wi th the absence of disease. Although our data hint toward a contribution of AIDA to ED-PWD, further studies will be necessary since the presence of the AIDA genes was also associated with the presence of the Shiga-like toxin a nd F18 adhesin genes, two reported virulence factors for ED-PWD.