Characterization and optimization of the exo beta 1-4 glucanase enzyme of the Aspergillus fumigatus 155 v cellulolytic fungus strain

An experiment using a completely randomized design with a 2x5x6 factorial a rrangement and 3 replications, was conducted to characterize the cellulase enzymes of the A. fumigatus 155 v cellulolytic fungus and to optimize the e nzyme production of the exo beta1-4 glucanase obtained from sugarcane bagas se fermentation after incubation for 0, 24, 48, 72, 96 and 120 hr and at 36 , 45, 50, 55 and 60 degreesC. Through a simple classification, the influenc e of the sodium citrate buffer pH (3.65, 4.19,4.80, 5.30 and 5.66) in the d etermination of the exo beta1-4 glucanase enzyme, was analysed as well as t heir optimum at different incubation periods (20, 30, 45, 60, 75 and 90 min utes) and the substrate concentrate to be degraded (10, 30, 50, 70 and 90 m g) by this enzyme. It was observed that the maximum cellulase enzyme expres sion of the A. fumigatus 155 v fungus was at 48 hr after the start of the s olid state fermentation process at all temperatures studied, as well as the performance of the exo beta1-4 glucanase enzyme at the different pH and pr oduction reached stability (2.7 IU/g DM) at between pH 5.7 and 5.6. The the rmostability was determined at 20 min and 60 degreesC and there was a maxim um expression of the enzyme of 8.9 IU/g for 70 mg of enzyme concentration. Thus, the kinetic parameters of the A. fumigatus 155 v strain were defined for the studies of the biotransformation of sugarcane bagasse through solid slate fermentation systems.