POLYSOMES FROM VICIA-FABA L LEAVES BOUND TO THE ACTIN CYTOSKELETON

The presence of polymeric actin in the polysome preparations from broa d bean leaves (Vicia faba L. var. Russkie chernye) was detected on dot blots and under the electron microscope using the phalloidin-colloida l gold marker. The fractions enriched in the cytoskeleton-bound polyso mes were isolated using different sensitivity of the membranes and cyt oskeletal elements to nonionic and ionic detergents. The distribution of a polypeptide with a molecular weight of about 40 kDa in the subcel lular fractions was studied and an enrichment of this polypeptide was observed in membrane-bound and especially cytoskeletal-bound polysomes . This polypeptide gave a positive signal when probed with anti-actin serum on Western blots.