Determination of synthesized alpha-vitamin E by micellar electrokinetic chromatography

We developed a micellar electrokinetic chromatography method (MEKC) for the direct determination of the content of synthesized alpha -vitamin E. It wa s found that under the optimum separation conditions 7 mM borate + 14 mM ph osphate + 15 mM sodium dodecyl sulfate (SDS) +10 mM sodium cholate (NaCh) 8% acetonitrile (pH 9.2) with UV detection wavelength at 214 nm, 16 kV con stant voltage, and 26 degrees C constant temperature, alpha -vitamin E and its isomers can be baseline separated and alpha -vitamin E was quantitative ly analyzed. In addition, the sample recovery, the limit of detection and t he repeatability of the method were investigated. The influence of various parameters on the separation such as SDS concentration, NaCh concentration, buffer pH and acetonitrile percentage were also discussed.