Development of a quality control material for the measurement of 8-oxo-7,8-dihydro-2 '-deoxyguanosine, an in vivo marker of oxidative stress, and comparison of results from different laboratories

Citation
J. Lunec et al., Development of a quality control material for the measurement of 8-oxo-7,8-dihydro-2 '-deoxyguanosine, an in vivo marker of oxidative stress, and comparison of results from different laboratories, FREE RAD RE, 33, 2000, pp. S27-S31
Citations number
10
Categorie Soggetti
Biochemistry & Biophysics
Journal title
FREE RADICAL RESEARCH
ISSN journal
10715762 → ACNP
Volume
33
Year of publication
2000
Supplement
S
Pages
S27 - S31
Database
ISI
SICI code
1071-5762(2000)33:<S27:DOAQCM>2.0.ZU;2-P
Abstract
The measurement of 8-oxo-7,8-dihydro-2'-deoxyguanosine is an increasingly p opular marker of in vivo oxidative damage to DNA. A random-sequence 21-mer oligonucleotide 5'-TCA GXC GTA CGT GAT CTC AGT-3' in which X was 8-oxo-guan ine (8-oxo-G) was purified and accurate determination of the oxidised base was confirmed by a P-32-end labelling strategy. The lyophilised material wa s analysed for its absolute content of 8-oxo-dG by several major laboratori es in Europe and one in Japan. Most laboratories using HPLC-ECD underestima ted, while GC-MS-SIM overestimated the level of the lesion. HPLC-ECD measur ed the target value with greatest accuracy. The results also suggest that n one of the procedures can accurately quantitate levels of 1 in 10(6) 8-oxo- (d)G in DNA.