Differences in binding characteristics of sex steroid binding protein in reproductive and nonreproductive female rainbow trout (Oncorhynchus mykiss),black bream (Acanthopagrus butcheri), and greenback flounder (Rhombosolea tapirina)
Ac. Hobby et al., Differences in binding characteristics of sex steroid binding protein in reproductive and nonreproductive female rainbow trout (Oncorhynchus mykiss),black bream (Acanthopagrus butcheri), and greenback flounder (Rhombosolea tapirina), GEN C ENDOC, 120(3), 2000, pp. 249-259
The binding characteristics of sex steroid binding protein (SBP) were inves
tigated in vitellogenic and nonreproductive female rainbow trout (Oncorhync
hus mykiss), black bream (Acanthopagrus butcheri), and greenback flounder (
Rhombosolea tapirina). The binding capacity of rainbow trout and black brea
m SBP was significantly greater in vitellogenic than in nonreproductive-sta
ge fish. A decrease in binding affinity was observed in male trout injected
with estradiol (E-2) compared to control fish. This difference was not obs
erved after partial purification of the SBP by gel filtration and may have
resulted from competitive inhibition of E-2 binding by vitellogenin. No dif
ferences in flounder SBP binding characteristics were observed. (C) 2000 Ac
ademic Press.