Serodiagnosis and treatment of listeriosis in repeat breeder cattle

Seroprevalence of listeriosis was studied in 75 repeat breeder and 15 pregn ant cattle kept as control by using standardized plate-ELISA and dot-ELISA using culture filtrate antigen of pathogenic Listeria monocytogenes. Seropo sitivity was recorded in 26 (34.6%) repeat breeder animals with titres rang ing from 1:200 to 1:3200 by plate-ELISA as compared to 28 (37.33%) animals by dot-ELISA. All the control animals were negative. Out of 26 positive rep eat breeders, 13 animals were treated with enrofloxacin each receiving 1.5g (i/m) for 3 days alongwith 300 mg drug intrauterine 24 hr post inseminatio n while 13 untreated animals were kept as control. Eleven (84.6%) out of 13 treated animals conceived as compared with none in untreated positive grou p.