Kinetoplast minicircle DNA analysis of Trypanosoma evansi by polymerase chain reaction using single oligonucleotide primers

Authors
Omanwar, S Rao, JR Singh, RK
Citation
S. Omanwar et al., Kinetoplast minicircle DNA analysis of Trypanosoma evansi by polymerase chain reaction using single oligonucleotide primers, I J ANIM SC, 71(1), 2001, pp. 5-7
Citations number
13
Categorie Soggetti
Animal Sciences
Journal title
INDIAN JOURNAL OF ANIMAL SCIENCES
ISSN journal
03678318 → ACNP
Volume
71
Issue
1
Year of publication
2001
Pages
5 - 7
Database
ISI
SICI code
0367-8318(200101)71:1<5:KMDAOT>2.0.ZU;2-B
Abstract
Kinetoplast DNA from Trypanosoma evansi isolates of bubaline, equine and ca meline origin were amplified by polymerase chain reaction (PCR) using 4 arb itrarily selected 10 to 11 base primers. Depending upon the T. evansi isola te-primer combination, between 3 to 8 reproducible DNA fingerprints of 300 to 1630 bp were amplified revealing a limited DNA polymorphism.