Factors affecting the production of (-)-mitorubrinic acid by Penicillium funiculosum

The optimum conditions for the production of (-)-mitorubrinic acid by mutan t strain of Penicillium funiculosum CCM F-8080 were examined in shaken flas k cultures. The highest production of this metabolite was, achieved in a me dium with glucose as the sole carbon source and (NH4)(2)HPO4 as the nitroge n source. The most suitable concentrations of the given sources for product ion of (-) -mitorubrinic acid are 80 g/l glucose and 2 g/l (NH4)(2)HPO4 (ma ximal production, 814.9 mg/l). We also observed the influence of concentrat ion of L-phenylalanine and inorganic phosphate in culture medium on the pro duction of (-)-mitorubrinic acid. The biological activity of mitorubrinic a cid as trypsin inhibitor was determined (IC50 41.05 mu mol/l).