The N-terminal N5 subdomain of the alpha 3(VI) chain is important for collagen VI microfibril formation

Citation
J. Fitzgerald et al., The N-terminal N5 subdomain of the alpha 3(VI) chain is important for collagen VI microfibril formation, J BIOL CHEM, 276(1), 2001, pp. 187-193
Citations number
32
Categorie Soggetti
Biochemistry & Biophysics
Journal title
JOURNAL OF BIOLOGICAL CHEMISTRY
ISSN journal
00219258 → ACNP
Volume
276
Issue
1
Year of publication
2001
Pages
187 - 193
Database
ISI
SICI code
0021-9258(20010105)276:1<187:TNNSOT>2.0.ZU;2-W
Abstract
Collagen VI assembly is unique within the collagen superfamily in that the alpha1(VI), alpha2(VI), and alpha3(VI) chains associate intracellularly to form triple hlelical monomers, and then dimers and tetramers, which are sec reted from the cell. Secreted tetramers associate end-to-end to form the di stinctive extracellular microfibrils that are found in virtually all connec tive tissues. Although the precise protein interactions involved in this pr ocess are unknown, the N-terminal globular regions, which are composed of m ultiple copies elf von Willebrand factor type A-like domains, are likely to play a critical role in microfibril formation, because they are exposed at both ends of the tetramers. To explore the role of these subdomains in col lagen VI intracellular and extracellular assembly, alpha3(VI) cDNA expressi on constructs with sequential N-terminal deletions were stably transfected into SaOS-2 cells, producing cell lines that express alpha3(VI) chains with N-terminal globular domains containing modules N9-N1, N6-N1, N5-N1, M4-N1, N3-N1, or N1, as well as the complete triple helix and C-terminal globular domain (C1-C5). All of these transfected alpha3(VI) chains were able to as sociate with endogenous (alpha1(VI) and alpha2(VI) to form collagen VI mono mers, dimers, and tetramers, which were secreted. Importantly, cells that e xpressed alpha3(VI) chains containing the N5 subdomain, alpha3(VI) N9-C5, N 6-C5, and N5-C5, formed microfibrils and deposited a collagen VI matrix. In contrast, cells that expressed the shorter alpha3(Vl) chains, N4-C5, N3-C5 , and N1-C5, were severely compromised in their ability to form end-to-end tetramer assemblies and failed to deposit a collagen VI matrix. These data demonstrate that the alpha3(VI) N5 module is critical for microfibril forma tion, thus identifying a functional role for a specific type A subdomain in collagen VI assembly.