The complete sequence of mtDNA genes in idiopathic dilated cardiomyopathy shows novel missense and tRNA mutations

Background: Previous studies have shown that mitochondrial DNA (mtDNA) muta tions are often present in patients with myocardial dysfunction. We sought to assess the prevalence and significance of heart mtDNA sequence changes i n patients with idiopathic dilated cardiomyopathy (DCM). Methods and Results: DNA sequence of all the transfer ribonucleic acid (tRN A), ribosomal RNA (rRNA), and structural genes in cardiac mtDNA of 28 patie nts with DCM was determined acid compared with a control group that had no evidence of heart disease. An increased number of point mutations were foun d in DCM cardiac mtDNA when compared with controls. Both novel and previous ly reported mutations were found in mitochondrial tRNA and structural genes . One of these mutations was heteroplasmic and resulted in changing a highl y conserved nucleotide in tRNA(Arg). Novel, heteroplasmic mtDNA mutations ( n = 4) specifying changes in moderate to highly conserved amino acid residu es were found in COII, COIII, ND5, and cytb. These novel mtDNA mutations we re found only in patients with severe reduction in mitochondrial enzyme act ivities. Conclusions: Our results indicate that a high incidence of mtDNA nucleotide sequence changes in both tRNA and structural genes are present in DCM. Fiv e heteroplasmic mutations were detected that both changed evolutionarily co nserved residues (which may impair the function of proteins or tRNAs) and w ere associated with specific enzymatic defects. These mutations could play an important role in the pathogenesis of cardiomyopathy.