High-performance liquid chromatographic separation of enantiomers of synthetic amino acids on a ristocetin A chiral stationary phase

A macrocyclic glycopeptide, ristocetin A, was used as chiral stationary pha se for the high-performance liquid chromatographic separation of enantiomer s of 28 unnatural amino acids, such as analogues of phenylalanine, tyrosine and tryptophan, and analogues containing 1,2,3,4-tetrahydroisoquinoline, t etraline or 1,2,3,4-tetrahydro-2-carboline skeletons. Excellent resolutions were achieved for most of the investigated compounds by using reversed-pha se or a new polar-organic mobile phase system. The conditions of separation were optimized by variation of the mobile phase composition, temperature a nd flow-rate. (C) 2000 Elsevier Science B.V. All rights reserved.