Fast enantioseparations of basic analytes by high-performance liquid chromatography using cellulose tris(3,5-dimethylphenylcarbamate)-coated zirconiastationary phases

In this work, we study the influence of the mobile phase and column tempera ture on the enantioresolution of basic compounds on microparticulate porous zirconia coated with cellulose tris(3,5-dimethylphenylcarbamate) (CDMPC). The chiral analytes are amino compounds, including a number of P-blockers. Analytes are eluted with hexane-alcohol mobile phases. We investigated the effect of alcohol (type and concentration), basic eluent additives, and col umn temperature on the parameters that control resolution (column efficienc y, retention and selectivity). Conditions for achieving an adequate separat ion in the least time have been determined for numerous racemic mixtures. F or most solutes, baseline resolution of the enantiomeric pair was achieved in less than 1 min; 12 of 13 pairs were separated in less than 2 min. (C) 2 000 Elsevier Science B.V. All rights reserved.