Quantitative determination of L-775,606, a selective 5-hydroxytryptamine 1D agonist, in rat plasma using automated sample preparation and detection by liquid chromatography-tandem mass spectrometry
Kl. Locker et al., Quantitative determination of L-775,606, a selective 5-hydroxytryptamine 1D agonist, in rat plasma using automated sample preparation and detection by liquid chromatography-tandem mass spectrometry, J CHROMAT B, 750(1), 2001, pp. 13-23
L-775,606 is under investigation as a selective 5-hydroxytryptamine 1D agon
ist for the treatment of migraine. A reliable and sensitive method for the
analysis of L-775,606 in plasma was required in order to support preclinica
l evaluation of this compound. A semi-automated sample preparation method u
sing the Beekman Biomek 2000 workstation to perform all liquid handling tas
ks has been established. The sample analysis was performed using HPLC-MS-MS
with a cycle time of 3.5 min per sample. Intra- and inter-day assay accura
cy and precision are excellent with a calibration range of 1-2000 ng/ml and
a reproducible limit of quantification of 1 ng/ml. (C) 2001 Elsevier Scien
ce B.V. All rights reserved.