Variation of African horsesickness virus nonstructural protein NS3 in southern Africa

NS3 protein sequences of recent African horsesickness virus (AHSV) field is olates, reference strains and current vaccine strains in southern Africa we re determined and compared. The variation of AHSV NS3 was found to be as mu ch as 36.3% across serotypes and 27.6% within serotypes. NS3 proteins of va ccine and field isolates of a specific serotype were found to differ betwee n 2.3% and 9.7%. NS3 of field isolates within a serotype differed up to 11. 1%. Our data indicate that AHSV NS3 is the second most variable AHSV protei n, the most variable being the major outer capsid protein, VP2. The inferre d phylogeny of AHSV NS3 corresponded well with the described NS3 phylogenet ic clusters. The only exception was AHSV-8 NS3, which clustered into differ ent groups than previously described. No obvious sequence markers could be correlated with virulence. Our results suggest that NS3 sequence variation data could be used to distinguish between field isolates and live attenuate d vaccine strains of the same serotype.