Chromatographic properties of reversed phase stationary phases under pressure and electro driven conditions. Effect of buffer composition

Four different reversed-phase (RP) stationary phases (CEC Hypersil C18, Zor bax Rx SIL C18, Zorbax 300 Rx SIL C18 and Zorbax PSM1000/C18) were examined under high-performance liquid chromatographic (pressure-driven, HPLC), and capillary electrochromatographic (electro-driven, CEC) conditions using an acetonitrile mobile phase combined with twenty different buffer systems (d ifferent cations, anions, pH and/or ionic strengths). Chromatographic perfo rmance tests under HPLC and CEC conditions were carried out using acidic, b asic and neutral polar/non-polar compounds. Parameters such as plate number , retention factor and asymmetry were used to describe the behavior of the RP-columns under both HPLC and CEC conditions. The buffer systems different ly influence chromatographic characteristics of porous RP-phases under CEC and HPLC conditions. Thus the choice of an appropriate buffer can be critic al for an application applied on an entire system. (C) 2000 John Wiley & So ns, Inc.