V. Kecskemeti et I. Balogh, The role of platelet-activating factor (PAF) antagonists and nitric oxide in cardiac actions of PAF. Electrophysiological and morphological study, J PHYSL PH, 51(4), 2000, pp. 723-735
Electrophysiological and ultrastructural effects of platelet-activating fac
tor (PAF) antgonists, WEB 2086 and BN 52021 were compared in isolated guine
a-pig hearts preparations. We studied the possible role of nitric oxide (NO
) in electromechanical actions of PAF. Isometric twitches and intracellular
action potentials (APs) were recorded from guinea-pig right ventricular pa
pillary muscles and left atria. For electron microscopic study the hearts w
ere perfused according to Langendorff technique. WEB 2086 (5 x 10(-9)-5 x 1
0(-7) M) significantly shortened the duration of atrial AP without changing
the ventricular one, however, BN 52021 decreased both of them. The shorten
ing of atrial and ventricular AP duration (APD) by both PAF antagonits were
abolished by 4-aminopyridine (10(-3) M), a blocker of one type of K+ chann
els (I-Kto). Glibenclamide (10(-6) M) the blocker of ATP-dependent K+ chann
els prevented the shortening effect of BN 52021 (10-6 M) on ventricular APD
. Electron microscopic study of myocardial samples from hearts subjected to
30 min hypoxia/reoxygenation showed intracellular oedema, intramitochondri
al swelling and fragmentation of mitochondrial christae, separation of inte
rcalated disc, Pretreatment with WEB 2086 (5 x 10(-7) M) warded off nearly
all damage caused by hypoxia/reoxygenation. Both WEB 2086 and NO synthase i
nhibitor N-G-nitro-L-arginine methyl esther (L-NAME) (10(-3) M) abolished t
he negative inotropic effect of PAF (10(-7), 10(-6) M). L-NAME prevented th
e shortening of APD induced by 10(-7) M PAF. These results suggest that PAF
may be responsible for myocardial ischemia and the beneficial effects of P
AF antagonists in this pathological process could be due to their possible
K+ channel stimulator property. data support the possibility that NO contri
butes to the cardiac electromechanical alterations induced by PAF.