Enzyme-linked immunosorbent assays of canine apolipoproteins B-100 and A-I

An enzyme-linked immunosorbent assay (ELISA) for canine blood apolipoprotei n (apo) B-100 and A-I was developed. The working range for the assay was 1. 8 to 28.7 ng/well for apoB-100 and it was 50 to 410 ng/well for apoA-I. The intra- and inter-assay coefficients of variation for the assay for apoB-10 0 were 5.4 and 6.9%, respectively, and for apoA-I they were 5.8 and 10.6%, respectively. The average concentrations of apoB-100 and A-I in 25 beagles (males, aged 3-4 years) were 0.084 +/- 0.028 (mean +/- SD) mg/ml and 6.29 /- 1.55 mg/ml, respectively. The ratios of canine (C) apoB-100 to CapoA-I w ere 1.41 +/- 0.58%. The respective concentrations in one case of hyperlipid emia with systemic atherosclerosis were 0.454 mg/ml and 11.28 mg/ml (a rati o of 4.03%). These values were larger than those of the controls. These res ults suggest that the measurements of CapoB-100 and A-I concentrations by t his newly developed ELISA are helpful for diagnosis of lipidosis.